-
April 2015, Have a look at the last release of Plos One, online Journal
The article presents the preparation and characterization of Phospho-(Tyrosine)-Mimetic Calmodulin Mutants.
Villalobo and al. prestents some very interesting properties after the characterisation of Phospho-(Tyrosine)-Mimetic Calmodulin Mutants.
>> READ MORE
Some physicochemical properties of these CaM mutants as compared to wild type one have been demonstrate:
1/ Some changes in their electrophoretic mobility in the absence and presence of Ca2+
2/ A ultraviolet (UV) light absorption spectra, far- and near-UV circular dichroism data
3/ A thermal stability in the absence and presence of Ca2+;
4/ A Tb3+-emitted fluorescence upon tyrosine excitation.
Some biochemical properties of these CaM mutants were also described such as their differential phosphorylation by the tyrosine kinase c-Src, and their action as compared to wild type, on the activity of two CaM-dependent enzymes: cyclic nucleotide phosphodiesterase 1 (PDE1) and endothelial nitric oxide synthase (eNOS) assayed in vitro.
Silviya R. Stateva, Valentina Salas, Gustavo Benaim, Margarita Menéndez, Dolores Solís, Antonio Villalobo, Characterization of Phospho-(Tyrosine)-Mimetic Calmodulin Mutants, Plos one, 2015, DOI: 10.1371.
-
February 2015, To Come a Themed Issue on Mutivalent Biomolecular Recognition by RSC Publisher - OBC (Organic & Biomolecular Chemistry)
OBC is delighted to announce a call for paper on Multivalent Biomolecular Recognition!
The submission deadline is 15th July 2015 for this themed issue. OBC submission
The 3 guest Editors: Dr. Mihai Barboiu (IEM, France), Prof Olof Ramström (KTH, Sweden) and Prof Stéphane Vincent (Univeristy of Namur, Belgium), all members of the EU-funded Dynanic Interactive Nanosystems ITN Marie Curie DYNANO Network, have been inspired for the themed issue in part by the area covered by DYNANO EU-project. Papers from colleagues of the network are indeed expected as well are welcome all articles from any contributors in the field who are not yet in the network.
{moshide hidden >> READ MORE | CLOSE <<}
Why Multivalent Biomolecular Recognition?
The anwser is given by the guest-editors:
The understanding and control of multivalent biomolecular interactions has become one of the key frontiers of chemistry and biology.
This issue has the aim to cover new discoveries and advances in the field in order to:
- Develop functionel sugar decorated nanoplatforms (nanoparticules, nanostructures surfaces, vesicles, microarrays etc..)
- Unterstand the biomolecular mechanisms by exploring their synergistic adaptative interactions with biomolecules, in order to accurately control the chemical/biological responses.
-
January 2015, Great release: Front Cover & Article in the latest issue of Chemical Communication!
Dynamic constitutional frameworks for DNA biomimetic recognition
Romina Catana, Mihai Barboiu et al. in collaboration with a
Romanian team present a linear and cross-linked
constitutional frameworks that show dominant coiling
DNA binding, closer to histone DNA Binding wrapping
mechanism.
>> READ MORE
Guanidium moiety of the linear or crossed linked
frameworks have shown a strong interaction with
the negative charge of backbone of the double stranded
DNA. This discovery leads the possibility of further more DNA systems to self-selected and self-generate the most adapted carrier for their own active transfection.
Dynamic Constitutional frameworks for DNA biomimetic recognition, Catana R., Barboiu M., Moleavin I., Clima L., Rotaru A. , Ursu EL. and Pinteala M., Chem. Comm., 2015, 51, 2021-2024.
ggg
gggg
ggg
ggg
ggg
ggg
ggg
ggg
-
December 2014: Have a look at the last release of the Journal of pharmacy and pharmaceutical Science!
Short review on the new axis in cancer therapy through the regulation exerted by the calcium-receptor protein calmodulin involving ErbB family receptor and the adaptator protein Grb7.
Villalobo et al. present a brief review with an overview and available information up-to-date on the research stage of Calmodulin regulation and its role inside the hyperactivity of both ErbB receptors and Grb7 in tumors. >> READ MORE To overcome problematic effects of CaM antagonists in cancer therapy. Potential new axis have been explored and presented here as targeting the site of action of CaM in CaM–binding systems to ErbB receptors and Grb7 protein. Both of them are found upregulated and /or overexpressed in tumors. A route to new potential anti-cancer therapy is open.
Targeting the Calmodulin-regulated ErbB/Grb7 Signaling Axis in Cancer Therapy, Villalobo A., Garcia-Palmero I., Stateva S. R., and Jellali K., J. Pharm. Pharmaceut. Sci., 2013, 16(2), 52-64.
ggg
-
December 2014: Have a look at the new release of JACS!
Facil access to dynamic covalent peptides by reverse Native Chemical Ligation (NCL)
Guiseppone's group in Strasbourg University has developed a new methodology of peptide exchange reaction without enzymatic catalysis. >> READ MORE
The exchange of peptide fragment can be achieved by chemoselective and reversible NCL with a high degree of control on the location of the reversible bond in dynamic peptides/ proteins (orthogonality to the other peptides bonds is respected). The reactions is efficient in mild conditions (Physiologic pH, aqueous media) in the presence of DTT, with only small modifications of cysteine residue (N-methyl-cys) compatible with large range of bioassays and beyond. The original NCL methodology can be extended to further peptide bonds as long as a thiol group is present or introduced. This new methodology to build dynamic covalent peptides is of a great interest and potential for the discovery or release of new bioactive molecules or development of dynamic functional materials.
Reversible Native Chemical Ligation: A facil Access to Dynamic Covalent Peptides, Y. Ruff; V. Garavini & N. Giuseppone, J. Am. Chem. Soc, 2014, 136, 6333-6339.
gg
-
June 2014: Do not miss the new Nature Communication!
The 1ST supramolecular artificial model to show natural mimicking Gramicidin-A (gA) channel!
The 1st route to conceive novel strategies for artificial channels mimicking nature (gA) was achieved by the collaboration between the NSA group at IEM, the ICGM team, both from Montpellier and the Fyles’ group from Victoria University, Canada.
>> READ MORE
Synthetic triazole forming TCT compounds called “T-channel” have shown good example of primitive channel mimicking the simplest known natural channel (Gramicidin-A).
Both theorical simulations and experiments reveal conduction through the T-channel similar to gA presenting proton/water conduction; cation/anion selectivity and a large open channel conductance states. Therefore, the association of supramolecular chirality with dipolar water alignment inside T-channels proved that they are a good model for artificial primitive mimic of gA! This discovery is of crucial relevance for the understanding of many biological events.
An artificial primitive mimic of the Gramicidin-A channel, Barboiu, M.; Le Duc, Y.; Gilles, A.; Cazade, P.-A.; Michau,M.; Legrand, Y.-M.; Van Der Lee, A.; Coasne, B.; Parvizi, P.; Post, J. & Files, T. M.; Nat. Comm., 2014,DOI:10,1038/ncomms 5142.
TOP
|
